1. When handling carbon dioxide cylinders, use hand carts, cranes, or overhead cranes for transportation.
2. Do not rely on twisting the protective ring to move the cylinders, as this can cause the cylinders to tip over, leading to potential injuries to personnel and equipment damage.
3. Ensure that the cylinders remain upright during operations to avoid tipping.
4. Never place horizontally or roll cylinders containing low-temperature carbon dioxide.
5. When loading cylinders onto a truck, use elevators or cranes to lift and position them onto the vehicle.
6. After loading the cylinders onto the truck, secure them in place using nylon straps. Avoid using chains for securing, as they can damage the surface of the cylinders and even compromise the vacuum integrity.

It is important to strictly adhere to these handling and transportation procedures to ensure the safety of personnel and the integrity of carbon dioxide cylinders throughout the process.